Recommended application field: Seta and Square dyes are untilized in Fluorescence Resonance Energy Transfer (FRET), or Fluorescence Lifetime (FLT) as a read-out parameter. These products are used in life sciences (biology, medicine and pharmacology), more specific areas onclude Biological Imaging, Cytology, Immunology, Drug Screening, Cellular and Molecular Biology, Proteomics, Genomics, High-throughput Screening, Photodynamic Therapy, Clinical Diagnostics.

Advantages over analogues: - Spectral properties. The Square and Seta dyes series absorb and emit in the 500-900 nm spectral range. Unlike the Cy and Alexa series, these red and NIR emitting markers can be excited not only with the red, 635- nm and 670- nm diode lasers but also with the blue, 370- nm or 405- nm lasers or light emitting diodes (LEDs). - Brightness. The red and NIR Square and Seta dyes have high extinction coefficients (up to 265,00 V[-1] cm[-1]) and protein conjugates of these labels are extremely bright (quantum yields up to 70%). - Photostability. Square and Seta dyes are in general more photostable compared to Cy or Alexa dyes. - Fluorescence Lifetimes. Square and Seta dyers are perfect tracers for lifetime (FLT) based assays. The microenvironment sensitive lufetimes of these dyes are in the range of 500 ps to 3 ns. We are currently also developing lifetime labels that have lifetimes in the order of 10 ns. - Sensitivity towards the microenvironment. Selected Square and Seta probes exhibit high affinity for proteins, biomembranes and lipoproteins and can be used to detectand quantitate these species. - Reactive Dark Quenchers. The newly developed reactive Dark Quenchers that absorb in the 600-800 nm spectral range have several times higher extinction coeficients as Black Hole Quenchers, do not exhibit any residual fluorescence and are perfectly suited for covalent labeling of proteins, peptides and oligonucleotides for use in FRET and real-time PCR based applications.

The development stage readiness: Tested experimental exploitation order

Description of the development:
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Proprietary fluorescent probes and labels of Square and Seta dyes series are used in biological and biomedical research, clinical disagnostics and high-throughput screening. These materials include: a) Reactive Red and nearinfrared (NIR) Fluorescent Labels for covalent attachment to biomolecules (proteins, fmino-acids, peptides, amino-modified oligonucleotides, DNA, RNA, etc.); b) Fluorescent Prodes for proteins, lipids and cells; c) Dark quenchers for Fluorescence Resonace Energy Transfer (FRET) Aplications; d) Classification Dyes (hydrophobic fluorophores) for single or multiple tncjding of microspheres used in High-throughput Screening (HTS).

Information about newness of the development:
there are Ukrainian patents -- 1 items

corresponds technical description
Conforms to technical characteristic

Possibility of transfer abroad:
Licence's sale

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Country Ukraine

For additional information turn to:
E-mail: gal@uintei.kiev.ua