Purpose of the development: DTprime for diagnostic laboratories requiring high throughput when performing routine analyzes and research institutions, where important opportunity to optimize and fine-tune.

Recommended application field: Medical, scientific and clinical research.

Technical characteristic: Specifications: The format of the fuser: • monoblock (version M1) - 96 vials of 0.2 ml (location 12x8) • 3-section fuser (modification M3) - 96 vials of 0.2 ml (location 12x8) • 6-cell fuser (option M6) - 96 vials of 0.2 ml (location 12x8) • monoblock (option X1) - 384 vials of 0.045 ml (location 24h16) Type of tube: • For modifications: PCR tubes 0.2 ml (individually or in strips of 8 pieces); 96-well microtiter plates for PCR 0.2ml • For modification X: 384-well microplate for PCR 0,045 ml Fuser temperature range from 0 to 100 ° C Readability setting temperatures 0.1 ° C Temperature stability of at least +/- 0.2 Average (maximum) rate of heating the fuser: • For modifications: 3,3 ° C / sec • To modify the X: 2,1 ° C / sec Average (maximum) rate of cooling the fuser: • For modifications: 2,1 ° C / sec • To modify the X: 1,0 ° C / sec Actuator Fuser: Peltier elements Excitation source: LED Detector: CCD The number of channels measuring fluorescence: 4 (option DTprime 4) 5 (modification DTprime 5) Spectral Range nm: 470-675 (modification DTprime 4) 470-750 (modification DTprime 5) Dimensions W x D x H: 210 x 540 x 540 Weight 27 kg

Advantages over analogues: Benefits of using DTprime: * High performance with 96-well format fuser, adapted to the standard plates, tubes and strips. * Multiplex detection with up to five channels of fluorescence and various combinations of fluorophores (in the base, DTpraym comes with four channels set to Hex, Fam, Cy5, Rox. The fifth channel can be installed by the customer). * Durability light sources (LEDs «LED»), which has a high operation duration (up to 100 000 hours), and stability compared with halogen lamps. * Optical path includes a separate light source for each channel, and CCD (CCD-camera). It provides high sensitivity, excellent signal / noise ratio and low interference between channels. * The method of multiple exposure provides a wide dynamic range of the detector, and allows at a qualitatively new level, to optimize the conditions for registration signal to simplify (or deselect all) photometric settings. * Optical system with different spectral characteristics allow you to work with multiple fluorophores. * Fuser allows you to set the gradient of the annealing temperature, opening additional opportunities for program optimization of amplification. * Standalone quest and recovery program (as a result otklyucheniyapitaniya or stop the computer) thanks to the integrated microcontroller and display. * Easy to manage and flexible settings are carried out with the help of the Russian-speaking software-based users with different levels of training. * It is possible to work with the program in a compact and in expanded mode, which provides a comfortable environment for clinical and research applications. * Ability to work with robotic sample preparation systems, thanks to the special geometry of the body and pull the fuser with automatic drive. * Ability to work as part of an integrated laboratory systems by storing data in a standard graphic and text formats, and its associated laboratory databases. * Increased productivity is ensured by the fact that several devices can be controlled by a single computer. In addition, results can be performed by the user on the computer during the amplification program. * Clear the area occupied by the device, it allows you to organize a complex of several devices. * The device is an open system and is not tied to specific reagents and plastic.

The development stage readiness: tested in laboratory condition

Description of the development:
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DTprime is the best choice for diagnostic laboratories requiring high throughput when performing routine analyzes and scientific institutions where important opportunity to optimize and fine-tune. The device is controlled software DTmaster c Russian / English user interface, common to all cyclers DT series with the detection of PCR results in real time. Software allows easy and intuitive operation using the function "Test", which significantly reduces the time an operator to establish protocols and minimizes the possibility of errors. Also, the software contains applications for the quantification of DNA / cDNA analysis of melting curves, determine the level of gene expression, study biocenoses and SNP-analysis allows to use the "additional standards" and create research protocols with automatic interpretation of the results.

corresponds technical description
Conforms to technical characteristic

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Country Ukraine

For additional information turn to:
E-mail: gal@uintei.kiev.ua