Purpose of the development: The development is intended to determine the activity of enzymes that can accelerate the hydrolysis of phosphorus-containing substrates.

Recommended application field: Clinical medicine, enzymology, microbiology, pharmacology, biochemistry.

Advantages over analogues: Compared to the best analogs, the sensitivity of the method improves to 0.2 U / L, the incubation time decreases to 20 minutes, and the time required for the formation of a colored substance to 1 minute. The technique is suitable for determining the content of orthophosphate in the presence of even the most labile phosphorus-containing substances, such as creatine phosphate.

The development stage readiness: tested in laboratory condition

Description of the development:
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The method for determining the activity of enzymes is based on spectrophotometric detection of orthophosphate after enzymatic hydrolysis of a phosphate-containing substrate, and the ionic associate of a 12-molybdophosphoric heteropolyanion with a polymethine dye is used as an analytical form for the determination of orthophosphate.

Information about newness of the development:
there are Ukrainian patents -- 1 items

corresponds technical description
Guarantees stable results getting

Possibility of transfer abroad:
Combinated reduction to industrial level

Photo

Country Ukraine

For additional information turn to:
E-mail: gal@uintei.kiev.ua