Purpose of the development: For micro fluorescence analysis

Recommended application field: Research, detection of ultra-low quantities of substances. Investigation of the photoluminescence surface of thin films, the detection of minute quantities of substances, the detection of single molecules, clusters and nanoparticles.

Technical characteristic: o spectral range - 400-950 nm; o spectral resolution - 1 nm; o area of the test sample - 100x100 mkm2; o spatial resolution - 1 mkm2; o the number of recording channels - 3-10-5; o Sensitivity - 100 photons / h; o dynamic range of> 104; o accumulation time up to 24 hours

Advantages over analogues: When using high quality components and thin-film samples (<1 micron) can identify the source of radiation with an accuracy better than 1 mkm3, enough to highlight the luminescence signal of a single molecule at a concentration in the matrix of the order of 1012 cm3.

The development stage readiness: Introduced in production

Description of the development:
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The principle of action is based on the excitation and emission registration molecules and nanostructures through one lens microscope using single-mode cw laser , the spatial separation of the radiation emitted by various elementary radiators , and spectral separation of the exciting radiation and luminescence . Image point radiators with spatial resolution of about 1 micron is investigated using a highly sensitive CCD array detectors (1024x300 elements) , cooled to a temperature of -120 ° C, which allows to record simultaneously and independently radiation of several molecules or molecular clusters with signal accumulation over an extended period ( to 24 hours). (CCD - Charge Coupled Device ) . For registration and study of the emission spectrum at the input of the CCD detector installed showing diffraction spectrograph . In this mode, analyzes 4- dimensional array: the coordinate (300 channels) , the wavelength ( channel 1024 ), the intensity (up to 216 samples) and time ( 105 seconds). These measurements allow us to study simultaneously and independently of the emission spectra of several molecules , clusters or nanoparticles , their dependence on the time and intensity of the exciting radiation dose . The system operates in equipment center for collective use IMAF NASB.

Possibility of transfer abroad:
Licence's sale
Technological document's sale
Realization of finished commodity

Photo

Country Belarus

For additional information turn to:
E-mail: gal@uintei.kiev.ua